Effects of Sperm DNA Fragmentation on Semen Parameters Effects of Sperm DNA Fragmentation on Semen Parameters and ICSI Outcome Determined by an Improved SCD Test, and ICSI Outcome Determined by an Improved SCD Test, Halosperm Halosperm

Abstract Background: Background: Sperm DNA fragmentation is known as an important cause of male infertility. Sperm DNA fragmentation is known as an important cause of male infertility. The influence of sperm DNA damage on reproductive potential has been subject of many studies The influence of sperm DNA damage on reproductive potential has been subject of many studies indicating various results and remaining the subject controversial. In this study, we investigated indicating various results and remaining the subject controversial. In this study, we investigated differences of the semen parameters and intracytoplasmic sperm injection (ICSI) outcome according differences of the semen parameters and intracytoplasmic sperm injection (ICSI) outcome according to sperm DNA fragmentation levels (DFLs) of patients. to sperm DNA fragmentation levels (DFLs) of patients. Materials and Methods: Materials and Methods: The DFLs were determined by Halosperm, a new improved sperm The DFLs were determined by Halosperm, a new improved sperm chromatin dispersion (SCD) test. Patients were grouped as low DNA fragmentation group (LFG chromatin dispersion (SCD) test. Patients were grouped as low DNA fragmentation group (LFG ≤30%) and high fragmentation group (HFG >30%). ≤30%) and high fragmentation group (HFG >30%). Results: Our analysis showed that semen parameters including concentration of untreated sperm and motility of prepared semen were low in HGF, whereas other parameters were not different. and motility of prepared semen were low in HGF, whereas other parameters were not different. Sperm DNA fragmentation levels decreased in both groups after semen preparation by density Sperm DNA fragmentation levels decreased in both groups after semen preparation by density gradient technique. gradient technique. Conclusion: Conclusion: No difference was detected on ICSI outcomes (fertilization, embryo development, No difference was detected on ICSI outcomes (fertilization, embryo development, embryo cleavage, embryo quality and pregnancy rates) between two group. embryo cleavage, embryo quality and pregnancy rates) between two group.