Regulatory effect of interleukin-10 and nitric oxide on activation of nuclear factor-κB and production of tumor necrosis factor in pulmonary alveolar macrophages induced by lipopolysaccharide

Objective:To evaluate the effects of interleukin10(IL10) and nitric oxide(NO) on the activation of nuclear factorκB(NFκB) and the expression of tumor necrosis factorα(TNFα) in pulmonary alveolar macrophages(PAM) induced by lipopolysaccharide.Methods:PAM collected by bronchusassociated lymphoid tissue(BALT) were cultured and divided into 4 groups:control group;lipopolysaccharide(LPS)stimulated group;IL10+LPS group and NO+LPS group.The NFκB activity of nuclear protein extract from the PAM and the concentration of TNFα in the supernatant were measured with electrophoretic mobility shift assay (EMSA) and enzyme linked immunoadsorbent assay(ELISA),respectively.Results:The activity of NFκB and the level of TNFα significantly increased at 3 hour after LPS stimulation;compared with LPS stimulated group,both NFκB activity and concentration of TNFα were significantly lowered in IL10+LPS group and NO+LPS group.Conclusions:LPS might activity NFκB in the PAM and induce an increase in transcription and expression of TNFα gene.IL10 and NO could inhibit the activation of NFκB and reduce the release of TNFα.