Effect of “Yiqi Kaimi Formula” on gastrointestinal motility in mice with slow transit constipation

Objective To observe the effects ofYiqi Kaimi Formulaon gastrointestinal motility in mice with slow transit constipation( STC). Methods Thirty models were established by injecting morphine and randomly divided into 3 groups: normal group,model group and Chinese medicine group. Each group was treated with corresponding interventions. We observed the percentage of carbon propelling,vasoactive intestinal peptide( VIP) and cajal cells markers after C-Kit immunostaining. Results Percentage of carbon propelling in the model group was decreased markedly( P 0. 05). Compared with the model group,the percentage of carbon propelling in Chinese medicine group was increased significantly( P 0. 05). After picture analyzing the positive area percentage of VIP,the positive nerve fibers in the model group was lower than that of the normal group,with significant difference between Chinese medicine group and model group( P 0.05). The C-Kit positive cells in model group were decreased,with significant difference between model group and normal group. It was also increased in Chinese medicine group,with significant difference between Chinese medicine group and model group( P 0. 05). Conclusion Yiqi Kaimi Formulahas positive effect on recovery of gastrointestinal motility in mice with STC induced by morphine. It may be association with promoting VIP expression and repairing the cells of Cajal.