ScholarLens Back to search
2010•Sichuan University of Arts and Science JournalRequires access

Study on Induction Condition in Fermentation of Escherichia Coli

Dan Liu

Open publisher page 0 citations

Abstract

By comparing the two counterpart groups of Fed-batch fermentations,in which one group is joined into the revulsant IPTG0.3mM and the other 0.5mM and induction time of one is 3h and the other 4h,observe the effects of recombinant protein expression to filter out the best condition of the high effective expression of target protein.Experiment shows that the best condition of protein expression is to add into the revulsant IPTG of the 0.5mM concentration in the middle and late growth of Escherichia Coli(OD600 is 4).

About this research paper

What this paper is about

By comparing the two counterpart groups of Fed-batch fermentations,in which one group is joined into the revulsant IPTG0.3mM and the other 0.5mM and induction time of one is 3h and the other 4h,observe the effects of recombinant protein expression to filter out the best condition of the high effective expression of target protein.Experiment shows that the best condition of protein expression is to add into the revulsant IPTG of the 0.5mM concentration in the middle and late growth of Escherichia Coli(OD600 is 4).

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

By comparing the two counterpart groups of Fed-batch fermentations,in which one group is joined into the revulsant IPTG0.3mM and the other 0.5mM and induction time of one is 3h and the other 4h,observe the effects of recombinant protein expression to filter out the best condition of the high effective expression of target protein.Experiment shows that the best condition of protein expression is to add into the revulsant IPTG of the 0.5mM concentration in the middle and late growth of Escherichia Coli(OD600 is 4).

Key concepts: Escherichia coli, lac operon, Fermentation, Recombinant DNA, Protein expression, Chemistry, Biology, Microbiology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Study on Induction Condition in Fermentation of Escherichia Coli — Research Paper | ScholarLens