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2019•bioRxiv (Cold Spring Harbor Laboratory)Open access

The roles of hox 13 genes in newt limb development and regeneration

Takashi Takeuchi, Fumina Minamitani, Kazuki Koriyama, Yukio Satoh, Kenichi Suzuki, Shuji Shigenobu, Takeshi Inoue, Kiyokazu Agata, Toshinori Hayashi

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Abstract

ABSTRACT Posterior Hox genes play crucial roles in limb development and specify regions in the proximal-distal (PD) axis of limbs. However, there is no direct genetic evidence that Hox genes are essential for limb regeneration. Moreover, if essential, it is totally unknown which Hox genes have the same or distinct functions between development and regeneration. Here, we mutated hox13 using an efficient CRISPR/Cas9 system in newts ( Pleurodeles waltl ), which have strong regenerative capacities in various tissues. Triple or double mutants of hox13 paralogs lost their digit and metacarpal/metatarsal bones. Limb regeneration progressed but regenerates lacked the same autopod region. These results showed that hox13 paralogs have the same functions in limb development and regeneration.

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ABSTRACT Posterior Hox genes play crucial roles in limb development and specify regions in the proximal-distal (PD) axis of limbs. However, there is no direct genetic evidence that Hox genes are essential for limb regeneration. Moreover, if essential, it is totally unknown which Hox genes have the same or distinct functions between development and regeneration. Here, we mutated hox13 using an efficient CRISPR/Cas9 system in newts ( Pleurodeles waltl ), which have strong regenerative capacities in various tissues. Triple or double mutants of hox13 paralogs lost their digit and metacarpal/metatarsal bones. Limb regeneration progressed but regenerates lacked the same autopod region. These results showed that hox13 paralogs have the same functions in limb development and regeneration.

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Available abstract

ABSTRACT Posterior Hox genes play crucial roles in limb development and specify regions in the proximal-distal (PD) axis of limbs. However, there is no direct genetic evidence that Hox genes are essential for limb regeneration. Moreover, if essential, it is totally unknown which Hox genes have the same or distinct functions between development and regeneration. Here, we mutated hox13 using an efficient CRISPR/Cas9 system in newts ( Pleurodeles waltl ), which have strong regenerative capacities in various tissues. Triple or double mutants of hox13 paralogs lost their digit and metacarpal/metatarsal bones. Limb regeneration progressed but regenerates lacked the same autopod region. These results showed that hox13 paralogs have the same functions in limb development and regeneration.

Key concepts: Hox gene, Limb development, Regeneration (biology), Biology, Pleurodeles, CRISPR, Anatomy, Gene

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