Effects of Resveratrol on Proliferation and Apoptosis of Endometrial Carcinoma AN3CA Cells and Its Mechanism

Objective: To investigate the effects of resveratrol(Res) on proliferation and apoptosis of endometrial cancer cell lines AN3CA and explore the involved mechanism in vitro.Methods: AN3CA cells were treated with different concentrations of resveratrol for various periods.The cell growth inhibitory rate was examined by MTT assay.The cell cycle arrest and apoptosis induction of resveratrol in AN3CA cells were detected by flow-cytometry analysis.We utilized real-time quantitative polymerase chain reaction(PCR) to identify expression level of Bcl-2,Bax and MMP-9 mRNA.The protein expression level of PCNA,Bcl-2,Bax,ERK1/2 and p-ERK1/2 were detected by Western-blot.Results: Resveratrol has significantly anti-proliferation effect on AN3CA cells which is in time-and concentration-dependent manners.It increases the G0/G1 phase cell population,whereas a concomitant decrease of cell numbers in S phase(P 0.01).In addition,resveratrol treatment could induce cellular apoptosis that the rate of apoptosis increased to 30.96%±2.041%(200μmol/l Res).Compared with the control,resveratrol treatment caused a decrease in the levels of PCNA and Bcl-2,whereas the level of Bax expression was upregulated.In a short time(0.5-1h),resveratrol can activate ERK1/2 phosphorylation but with the time prolonged,it showed inhibition of the expression of p-ERK.Conclusion: It seems that resveratrol has the antitumor action including inhibition of cell proliferation,blocking cells at the G0/G1 phase and inducing apoptosis.It regulated expression level of apoptosis-related Bcl-2 family protein.The antitumor effect of resveratrol is possiblly mediated by ERK1/2 pathway.