Effects of panax notoginsenosides on bone histomorphometry in rats with hyperlipidemia-induced osteoporosis

AIM:To explore the effects of panax notoginsenosides(PNS)on bone histomorphometry in rats with hyperlipidemia-induced osteoporosis.METHODS:Thirty 3-month-old Sprague-Dawley rats were randomly divided into three groups with 10 per group as follows.Control group was intragastrically given distilled water at the dose of 5 mL·kg-1·d-1 for 20 weeks;Model group was intragastrically given lipid emulsion at the dose of 5 mL·kg-1·d-1 for 20 weeks;PNS preventing group was intragastrically given lipid emulsion at the dose of 5 mL·kg-1·d-1 in the morning and PNS at the dose of 5 mL·kg-1·d-1 at afternoon for 20 weeks.At the end of the experiment,the right longitudinal proximal tibial metaphyseal parameters of rats were performed undecalcifiedly and used for bone histomorphometry analysis.And the blood serum contents of FFA,TG,TC,HDL-C and LDL-C were measured by biochemical analysis.RESULTS:Compared with model group,the blood serum contents of FFA,TC and LDL-C were decreased and HDL-C was increased significantly(P0.01),the PTM cancellous bone of %Tb.Ar,Tb.Th and Tb.N were increased(P0.01)and Tb.Sp was decreased significantly(P0.01).The dynamic parameters in cancellous bone of %L.Pm and bone formation rate(BFR/BS,BFR/BV)were increased(P0.05).CONCLUSION:Long-term gastric perfusion of lipid emulsion could induce hyperlipidemia-induced osteoporosis,and PNS could partly prevent it.