Research on enzyme production by Aspergillus oryzae 3.042 in solid state fermentation and enzyme activity

The cultivation conditions for the production of enzyme by Aspergillus oryzae 3.042 under solid-state fermentation were investigated.The effects of carbon and nitrogen sources,initial pH and fermentation temperature of culture on enzyme production were studied.The results showed that the optimum carbon source was wheat bran,the optimum nitrogen source was peptone.The optimal initial pH and temperature was 6.0～8.0 and 30℃,respectively.Furthermore,the determination of enzyme system and enzymatic activity for the main enzymes was analyzed,such as protease,xylanase and cellulase.This analysis provided theory foundation for research and application of enzyme-system from Aspergillus Oryzae in future industry.