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2014•Zhongguo yaoke daxue xuebaoRequires access

Effects of H 2 S on tetrodotoxin-sensitive sodium currents in mouse dorsal root ganglion neurons

Xian Wenyin

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Abstract

The effects of hydrogen sulfide( H 2 S) on tetrodotoxin-sensitive( TTX-S) sodium currents in mouse dorsal root ganglion( DRG) neurons,were studied by use of the whole-cell patch clamp technique. NaHS( a donor of H 2 S) increased TTX-S sodium currents in a concentration-dependent manner; the EC 50 was 119 μmol/L and Hill coefficient was 1. 105. NaHS 100 μmol/L markedly shifted the steady state activation and inactivation curves of TTX-S sodium currents towards more positive potential to 9. 8 and 10. 4 mV,respectively,yet with no significant difference on recovery from inactivation of TTX-S sodium channels. Therefore,H 2 S increased the TTX-S sodium currents and altered the activation and the inactivation kinetics of TTX-S sodium channel,which may contribute to one of its mechanisms in pain.

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What this paper is about

The effects of hydrogen sulfide( H 2 S) on tetrodotoxin-sensitive( TTX-S) sodium currents in mouse dorsal root ganglion( DRG) neurons,were studied by use of the whole-cell patch clamp technique. NaHS( a donor of H 2 S) increased TTX-S sodium currents in a concentration-dependent manner; the EC 50 was 119 μmol/L and Hill coefficient was 1. 105. NaHS 100 μmol/L markedly shifted the steady state activation and inactivation curves of TTX-S sodium currents towards more positive potential to 9. 8 and 10. 4 mV,respectively,yet with no significant difference on recovery from inactivation of TTX-S sodium channels. Therefore,H 2 S increased the TTX-S sodium currents and altered the activation and the inactivation kinetics of TTX-S sodium channel,which may contribute to one of its mechanisms in pain.

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Available abstract

The effects of hydrogen sulfide( H 2 S) on tetrodotoxin-sensitive( TTX-S) sodium currents in mouse dorsal root ganglion( DRG) neurons,were studied by use of the whole-cell patch clamp technique. NaHS( a donor of H 2 S) increased TTX-S sodium currents in a concentration-dependent manner; the EC 50 was 119 μmol/L and Hill coefficient was 1. 105. NaHS 100 μmol/L markedly shifted the steady state activation and inactivation curves of TTX-S sodium currents towards more positive potential to 9. 8 and 10. 4 mV,respectively,yet with no significant difference on recovery from inactivation of TTX-S sodium channels. Therefore,H 2 S increased the TTX-S sodium currents and altered the activation and the inactivation kinetics of TTX-S sodium channel,which may contribute to one of its mechanisms in pain.

Key concepts: Dorsal root ganglion, Sodium channel, Chemistry, Tetrodotoxin, Sodium, Biophysics, Patch clamp, Ganglion

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Effects of H 2 S on tetrodotoxin-sensitive sodium currents in mouse dorsal root ganglion neurons — Research Paper | ScholarLens