Activation of the ambusher tick Rhipicephalus microplus (Acari: Ixodidae) exposed to different stimuli

Abstract The present study aimed to evaluate the behaviour of larvae of Rhipicephalus microplus exposed to different stimuli. A Y‐olfactometer was positioned vertically and R. microplus larvae were exposed to environmental air, CO 2 alone, N , N ‐diethyl‐3‐methylbenzamide (DEET) alone, and CO 2 combined with the repellents DEET and ( E )‐2‐octenal. Tests were also conducted with the olfactometer positioned horizontally; in this case, however, only CO 2 was tested. In all tests conducted with the Y‐olfactometer positioned vertically, CO 2 activated R. microplus larvae even in the presence of DEET and ( E )‐2‐octenal, although activation was lower when these repellents were used. In the absence of CO 2 , larval behaviour against DEET was similar to that of the larvae in the control group. In the tests performed with the olfactometer positioned horizontally, the larvae had no significant response to the presence of CO 2 . The larvae were not attracted to or repelled by any compound tested in either the vertical or horizontal position of the olfactometer. The lack of horizontal displacement, attraction or repellence may have been a result of the ambush behaviour of this tick species. However, when larvae were exposed to stimuli and the olfactometer was positioned vertically, the interference of attractant and repellent stimuli in larval behaviour was assessed.