Flavobacterium jocheonensis sp. nov., Isolated from Marine Green Alga Ulva pertusa

A bacterial strain, labeled UR11 T , was isolated from green alga Ulva pertusa collected from Jeju Island, Korea.UR11 T was identified as a gram-negative, rod-shaped, motile by gliding and aerobic bacterial strain with yellow colonies on R2A plates.The strain UR11 T grew over at a temperature range of 10°C to 30°C (optimally at 25°C), a pH range of 6.0-11 (optimally at pH 7.0) and a Nacl range of 0.5-5% Nacl (w/v).Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain UR11 T was a member of the genus Flavobacterium.Strain UR11 T shared close similarity with F. jejuensis EC11 T (98.0%)F. jumunjinense HME7102 T (96.1%),F. haoranii LQY-7 T (95.3%),F. dongtanense LW30 T (95.1%), and F. ahnfeltiae 10Alg 130 T (94.9%).The major fatty acids (>5%) were iso-C 15:0 (33.9%), iso-C 15:1 G (12.4%), iso-C 17:0 3-OH (9.0%), iso-C 16:0 (7.0%) and iso-C 15:0 3-OH (6.3%).The major polar lipids were phosphatidylethanolamine, seven unknown aminolipids, two unknown aminopolarlipids and two unknown lipids.DNA-DNA hybridization value was 58% at strain UR11 T with F. jejuensis EC11 T .Based on phenotypic, chemotaxonomic and phylogenetic evidence, strain UR11 T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium jocheonensis sp.nov. is proposed.The type strain is Flavobacterium jocheonensis is UR11 T (=KCTC 52377 T =JCM 31512 T ).