Studying Antibiotic-Producing Bacteria in Leawood Soil

Thirteen antibiotic producing bacterial colonies found in soil from a Leawood, KS backyard from serial dilutions were isolated on master plates and tested against 5 safe relatives of the ESKAPE pathogens (which are relatives of highly resistant bacterial species). One of these colonies presented inhibition against Acintobacter baylyi. This antibiotic-producing bacteria, renamed #8, is the focus of continuing study. A quadrant streak plate of bacteria #8, with individual colonies, was used to creating a Gram stained slide. Examination of the slide revealed the bacteria’s Gram-negative status and appearance as short bacillus. It will be undergoing sequencing to verify its genus and metabolic testing to discover additional biochemical characteristics.