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Cloning and sequencing of the S1 spike proten gene of the infectious bronchitis virus

Chen Li-jun, Ying Wang, Jianhua Hu, Zhou Zongqin, Jianrong Wang

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Abstract

S1 spike protein is one of the important functional proteins fo infectious bronchitis virus (IBV). Based on the reported sequence of the spike protein gene, IBV-Beaudette, two specific primers were synthesized. A 1652 bp DNA fragment was amplicated by RT-PCR from the viral RNA of IBV, Shanghai isolate S_(2)T_(2), and further cloned into plasmid pSK(+). Restriction mapping revealed that the result was consistent with the reported data. Comparison of sequence fo S1 spike gene of S_(2)T_(2) with IBV-Beaudette, -Japan isolate, -M41 showed that the homologies were 95.1%、93.6% and 96.7% in amino acid respectively.

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What this paper is about

S1 spike protein is one of the important functional proteins fo infectious bronchitis virus (IBV). Based on the reported sequence of the spike protein gene, IBV-Beaudette, two specific primers were synthesized. A 1652 bp DNA fragment was amplicated by RT-PCR from the viral RNA of IBV, Shanghai isolate S_(2)T_(2), and further cloned into plasmid pSK(+). Restriction mapping revealed that the result was consistent with the reported data. Comparison of sequence fo S1 spike gene of S_(2)T_(2) with IBV-Beaudette, -Japan isolate, -M41 showed that the homologies were 95.1%、93.6% and 96.7% in amino acid respectively.

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Available abstract

S1 spike protein is one of the important functional proteins fo infectious bronchitis virus (IBV). Based on the reported sequence of the spike protein gene, IBV-Beaudette, two specific primers were synthesized. A 1652 bp DNA fragment was amplicated by RT-PCR from the viral RNA of IBV, Shanghai isolate S_(2)T_(2), and further cloned into plasmid pSK(+). Restriction mapping revealed that the result was consistent with the reported data. Comparison of sequence fo S1 spike gene of S_(2)T_(2) with IBV-Beaudette, -Japan isolate, -M41 showed that the homologies were 95.1%、93.6% and 96.7% in amino acid respectively.

Key concepts: Infectious bronchitis virus, Avian infectious bronchitis virus, Spike Protein, Biology, Gene, Cloning (programming), Virology, Plasmid

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Cloning and sequencing of the S1 spike proten gene of the infectious bronchitis virus — Research Paper | ScholarLens