2009•Guizhou nongye kexueRequires access
Study on SSR marker polymorphism and molecular classification of twelve waxy maize self-lines.
Ang LiuQi, Wenpeng Yang, Mingchun Wang, Hong Ren
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Abstract
The DNA of twelve waxy maize self-lines bred by Guizhou Institute of Upland Crops was determined by 30 SSR markers to study allelic variation of these SSR markers loci and the blood relationship between 12 tested waxy maize self-lines.The result showed that 95 alleles were determined in 12 tested self-lines,the range of determined allele from a pair of primers was from 1 to 9 and its average value was 3.17,which indicated high genetic diversity.Twelve waxy maize self-lines were divided into 3 groups by the cluster analysis and 4 lines(QCL5024,QCL5036S,QCL5036B and QCL5037)from the same hybrid combination could be classified into one group,which expresses that the result can provide a reference in hereditary and breeding of waxy maize.