SCREENING THE POTENTIAL OF BIOACTIVE PROTEIN AS ANTICANCER AGENTS FROM EPIPHYTIC BACTERIA ASSOCIATED WITH BROWN ALGAE, SARGASSUM SP

The exploration of bioactive protein from epiphytic bacteria associated with marine algae has not much been revealed.One of the symbiotic bacteria producing bioactive protein was successfully isolated from brown algae Sargassum sp.Molecular identification of bacteria was done by using PCR 16S rRNA sequencing.Based on the results, the active strains of bacteria isolate SG-A1 belonging to Enterobacter hormaechei with 96% sequence similarities.The crude extract protein of symbiotic bacterial isolates then was determined for the toxicity assay by using the Artemia salina Leach.The optimum time to produce bioactive proteins was evaluated.Enterobacter hormaechei showed the highest bioactivity at the time fermentation of 24 h with LC50 is 1,73 µg/mL.These findings suggest that the identified strains are very potential as protein sources for anticancer agent.Further research will be carried out using the optimum time for producing bioactive protein and more purification.