Quantitative phase imaging with digital holographic microscopy applied for label-free analysis of confluent cell layers
Björn Kemper, Luisa Pohl, Mathias Kaiser, Jürgen Schnekenburger, Steffi Ketelhut
Abstract
Björn Kemper, Luisa Pohl, Mathias Kaiser, Jürgen Schnekenburger, Steffi Ketelhut
Abstract
We explored strategies to analyze confluent cell layers utilizing digital holographic microscopy (DHM). Our results show that histogram-based evaluation of quantitative DHM phase images enables a highly reliable quantitative observation of global morphology changes.
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We explored strategies to analyze confluent cell layers utilizing digital holographic microscopy (DHM). Our results show that histogram-based evaluation of quantitative DHM phase images enables a highly reliable quantitative observation of global morphology changes.
Key concepts: Digital holographic microscopy, Phase imaging, Microscopy, Holography, Digital holography, Histogram, Phase (matter), Optical microscope