2019•Unpublished venueRequires access

Quantitative phase imaging with digital holographic microscopy applied for label-free analysis of confluent cell layers

Björn Kemper, Luisa Pohl, Mathias Kaiser, Jürgen Schnekenburger, Steffi Ketelhut

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Abstract

We explored strategies to analyze confluent cell layers utilizing digital holographic microscopy (DHM). Our results show that histogram-based evaluation of quantitative DHM phase images enables a highly reliable quantitative observation of global morphology changes.

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What this paper is about

We explored strategies to analyze confluent cell layers utilizing digital holographic microscopy (DHM). Our results show that histogram-based evaluation of quantitative DHM phase images enables a highly reliable quantitative observation of global morphology changes.

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Available abstract

We explored strategies to analyze confluent cell layers utilizing digital holographic microscopy (DHM). Our results show that histogram-based evaluation of quantitative DHM phase images enables a highly reliable quantitative observation of global morphology changes.

Key concepts: Digital holographic microscopy, Phase imaging, Microscopy, Holography, Digital holography, Histogram, Phase (matter), Optical microscope

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