2019•Sustainable Forestry CollectionRequires access
The study on callus induction from different explants of Myrica nana A. Chev.
Hou Lin-lin
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Abstract
In order to establish the suspension cell line of Myrica rubra to extract the effective components such as polyphenols,flavonoids, quercetin and dihydromyricetin. Effect of different plant growth regulator on root, stalk and leaf callus induction was studied in this paper. It showed that optimal root callus induction culture medium was MS + 0.2 mg ∙L −1 6-BA + 1mg ∙L −1 2 ， 4-D ,optimal stalk callus induction culture medium was MS + 0.6 mg ∙L −1 6-BA + 1mg ∙L −1 2 ， 4-D, Optimal stalk callus induction culture medium was MS + 0.2 mg ∙L −1 6-BA + 3mg ∙L −1 2 ， 4-D + 0.2mg ∙L −1 NAA. Root, stalk and leaf callus induction rate were 100% 、 78.67% and 21.3% in their optimum culture conditions respectively.