Lipase-catalyzed regio- and stereoselective deacylation: Separation of anomers of peracylated α,β-D-ribofuranosides

Efficient regio- and stereoselective deacylation of acyloxy function involving C-5' hydroxyl group of α-anomer over the other similar C-5' acyl group of β-anomer and acyl groups involving secondary hydroxyls in anomeric mixture of peracylates of D-ribose has been achieved during deacylation reaction mediated by Lipozyme® TL IM (Thermomyces lanuginosus lipase immobilized on silica). This enzymatic methodology has been efficiently used for the separation of anomeric mixtures of peracylated α,β-D-ribofuranosides.