2018•Unpublished venueRequires access

Antagonistic Activity of Trichoderma Strains Against Pathogenic Fungi of Arachis hypogaea L.

Khin Yuzana, Hla Min Thein

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Abstract

Fungi were isolated from the rhizospheric soils and infected leaves of Arachis hypogaea L. (groundnut) collected from Shwe Kyethtauk village, Sagaing Township, Sagaing Region. The fungi were isolated from rhizospheric soil using the Rose Bengal Medium for (KYZN 01), the Potato Dextrose Agar (PDA) medium for (KYZN 02) and (KYZN 03), from the infected leaves using the PDA medium. Pathogenic fungi were isolated from the infected parts of groundnut such as root rot, crown rot and pod rot by using PDA medium. The resulting pathogenic fungi were confimed (KYZN 04) as Macrophomina sp., (KYZN 05) as Aspergillus sp. and (KYZN 06) as Fusarium sp.. The assay for antagonism was performed on PDA medium by dual culture method. The maximum inhibitory activity of Trichoderma strain (KYZN 01) was 69% against Macrophomina sp. and followed by 66% on Fusarium and 50% on Aspergillus sp.. Trichoderma strain (KYZN 02) showed maximum inhibition against 61% on Macrophomina sp. and followed by 50% on Aspergillus sp. and 48% on Fusarium sp. Trichoderma strain (KYZN 03) showed the highest effects 72% against Macrophomina sp. but minimum inhibition was in 60% on Fusarium sp. and 56% on Aspergillus sp.. The significant inhibition of all three strains were observed in Macrophomina sp.. KYZN 03 was maximum inhibition percentage against on the all pathogenic fungi. All of the Trichoderma strains have significantly inhibition on pathogenic fungi which induce the major diseases symptom of groundnut plants. Trichoderma strains can be used as effective biocontrol agents for the diseases of groundnut plants.

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What this paper is about

Fungi were isolated from the rhizospheric soils and infected leaves of Arachis hypogaea L. (groundnut) collected from Shwe Kyethtauk village, Sagaing Township, Sagaing Region. The fungi were isolated from rhizospheric soil using the Rose Bengal Medium for (KYZN 01), the Potato Dextrose Agar (PDA) medium for (KYZN 02) and (KYZN 03), from the infected leaves using the PDA medium. Pathogenic fungi were isolated from the infected parts of groundnut such as root rot, crown rot and pod rot by using PDA medium. The resulting pathogenic fungi were confimed (KYZN 04) as Macrophomina sp., (KYZN 05) as Aspergillus sp. and (KYZN 06) as Fusarium sp.. The assay for antagonism was performed on PDA medium by dual culture method. The maximum inhibitory activity of Trichoderma strain (KYZN 01) was 69% against Macrophomina sp. and followed by 66% on Fusarium and 50% on Aspergillus sp.. Trichoderma strain (KYZN 02) showed maximum inhibition against 61% on Macrophomina sp. and followed by 50% on Aspergillus sp. and 48% on Fusarium sp. Trichoderma strain (KYZN 03) showed the highest effects 72% against Macrophomina sp. but minimum inhibition was in 60% on Fusarium sp. and 56% on Aspergillus sp.. The significant inhibition of all three strains were observed in Macrophomina sp.. KYZN 03 was maximum inhibition percentage against on the all pathogenic fungi. All of the Trichoderma strains have significantly inhibition on pathogenic fungi which induce the major diseases symptom of groundnut plants. Trichoderma strains can be used as effective biocontrol agents for the diseases of groundnut plants.

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Available abstract

Fungi were isolated from the rhizospheric soils and infected leaves of Arachis hypogaea L. (groundnut) collected from Shwe Kyethtauk village, Sagaing Township, Sagaing Region. The fungi were isolated from rhizospheric soil using the Rose Bengal Medium for (KYZN 01), the Potato Dextrose Agar (PDA) medium for (KYZN 02) and (KYZN 03), from the infected leaves using the PDA medium. Pathogenic fungi were isolated from the infected parts of groundnut such as root rot, crown rot and pod rot by using PDA medium. The resulting pathogenic fungi were confimed (KYZN 04) as Macrophomina sp., (KYZN 05) as Aspergillus sp. and (KYZN 06) as Fusarium sp.. The assay for antagonism was performed on PDA medium by dual culture method. The maximum inhibitory activity of Trichoderma strain (KYZN 01) was 69% against Macrophomina sp. and followed by 66% on Fusarium and 50% on Aspergillus sp.. Trichoderma strain (KYZN 02) showed maximum inhibition against 61% on Macrophomina sp. and followed by 50% on Aspergillus sp. and 48% on Fusarium sp. Trichoderma strain (KYZN 03) showed the highest effects 72% against Macrophomina sp. but minimum inhibition was in 60% on Fusarium sp. and 56% on Aspergillus sp.. The significant inhibition of all three strains were observed in Macrophomina sp.. KYZN 03 was maximum inhibition percentage against on the all pathogenic fungi. All of the Trichoderma strains have significantly inhibition on pathogenic fungi which induce the major diseases symptom of groundnut plants. Trichoderma strains can be used as effective biocontrol agents for the diseases of groundnut plants.

Key concepts: Arachis hypogaea, Biology, Soil fungi, Botany, Microbiology

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Antagonistic Activity of Trichoderma Strains Against Pathogenic Fungi of Arachis hypogaea L. — Research Paper | ScholarLens