The Genetic Transformation System in Model Species Physcomitrella patens

Gene-targeting efficiency in the land plant Physcomitrella patens(Bryophyta) can only be compared with that observed in Saccharomyces cerevisiae.Physcomitrella patens,as the new green yeast,might well become a major tool for functional genomic studies of multicellular eukaryotes.In addition,the relatively simple developmental pattern and the haploid gametophyte in the life history make it a suitable genetic tool.Molecular tools and genetic information are rapidly developing for P.patens.The current knowledge of Physcomitrella patens transformation system including the construction of vector,the transformation method and the preparation of the host cells are reviewed.The application of the Physcomitrella patens genetic transformation system was exampled at the last.