Screening of Antimicrobial Activity of Bacillus Species Isolated From Soil

Most antibiotics used today for the treatments of infectious diseases caused by pathogenic bacteria are mostly isolated from microbes and the increase in emergence of resistance need broad spectrum antibiotics are required nowadays. Aim of this study is the extraction of new antibacterial compounds from soil samples and determination of their efficacy against human pathogenic bacteria. The present study was designed to screened antibiotic production by Bacillus species, supernatant of isolates was taken and antibacterial activity was tested against gram positive bacteria including S. aureus, Micrococcus and gram negative bacteria including Pseudomonas, E.coli and Proteus by using agar well diffusion method. Isolated strain shows zone of inhibition against S. aureus, Micrococcus, E.coli, and Proteus. No zone of inhibition has been shown against Pseudomonas. Biochemical analysis was performed for the identification of Bacillus species. For further investigation, identification of strains may be helpful in improving antibiotic production.