Assessment of Phytochemical Content and Antioxidant Activity of the Extracts Collected from the Leaves and Flowers of Hypericum scabrum

To study the bioactive substances of the H. scabrum, ethanolic and aqueous extracts of leaves and flowers were analyzed for their phytochemical contents and antioxidant actings. The antioxidant potiential was estimated using four methods, including DPPH radical, ABTS decolorization assay, reducing power and total antioxidant capacity. The results exhibited that the ethanolic flower contain higher amounts of total flavonoids (4.675 mg. g-1) and saponin (29.572  mg.g -1) contents, whereas, the water flower extract hold less amount of total flavonoids compounds (1.223 mg. g-1). Similarly, our results exposed that the antioxidant activities detected by DPPH and ABTS methods of the ethanolic extract of flower, were evidently better than the rest of extracts. Significant high relationship were noticed between total phenolic amount and total antioxidant activity, and between total flavonoid content and DPPH inhibition assay. Principal component analysis reveals 91.49 of total variance which indicates that the different extracts were well distinguished by their  phytochemical substance contents and antioxidant effects.