2019•DEStech Transactions on Environment Energy and Earth ScienceRequires access
Inducing Adventitious Buds Regeneration from Leaf Explants of Purple Coneflower (Echinacea Purpurea L.)
Bing-zhen LIU, Hongjie Liu, Tingting Xie, Kai-zhe LIU, Xiaopeng Li, Guangjian Liu, Yingbin Xue, Miao Chen, Ying Liu, Shaoxia Yang
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Abstract
In the present study, purple coneflower leaves were used as explants to explore the effects of basic medium, CuSO4, and Diethyl aminoethyl hexanoate (DA-6) on induction of adventitious buds. The results shown that MS medium was the most suitable basic medium for inducing adventitious buds regeneration from leaf explants. When the concentration of CuSO4 was at 0.5 mg/L, a great effect of induction of adventitious buds was obtained (90%). Meanwhile, the optimal culture condition for the proliferation of adventitious buds was MS medium containing 0.5 mg/L 6-benzylaminopurine (6-BA).