2007•Natural Product CommunicationsOpen access

Application of High-Performance Liquid Chromatography for Simultaneous Identification of Integristerone A, 20-Hydroxyecdysone, Ecdysone and 2-Deoxy-20-hydroxyecdysone

Viatcheslav Rybin, Eugene Boltenkov, Е. В. Новожилова

Abstract

Conditions for separation and identification of integristerone A (1), 20-hydroxyecdysone (2), ecdysone (3) and 2-deoxy-20-hydroxyecdysone (4) by reversed-phase HPLC with UV and mass-selective detection at atmospheric pressure chemical ionization have been developed. Correlation between quasi-molecular ions fragmentation and structure peculiarities of the studied ecdysteroids has been shown. Application of these conditions for the analysis of extract from the inflorescences of Serratula komarovii made it possible to reveal and to identify compounds 1, 2 and 4.

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Key concepts: Ecdysone, 20-Hydroxyecdysone, Chromatography, High-performance liquid chromatography, Chemistry, Ecdysteroid, Fragmentation (computing), Biology