Accelerated and efficient method for isolating microRNA from human blood plasma

He study is dedicated to solving the problem of microRNA extraction from human blood for further use of the microRNA profile in the diagnosis of various diseases. The aim of the work is to choose an effective, accelerated method for the isolation of microRNA from human blood plasma, sufficient in quantity for diagnosing in clinical practice. Comparison of three methods for obtaining microRNA was carried out: 1) using a silicon oxide-based QIAamp RNA Blood Mini Kit column, 2) phenol-chloroform extraction using TRIzol LS Reagent and 3) phenol-chloroform extraction using TRIzol Reagent. It was established that for the most complete isolation of total human RNA from human blood plasma in order to obtain microRNA, TRIzol LS Reagent can be efficiently used. Because its use allows obtaining microRNA in less time - 30-40 min, and in an amount sufficient for clinical analysis (total RNA - up to 0,116 ng/μl, and microRNA - 350,3 picograms/μl), and also with the least amount of concomitant messenger RNA and long non-coding RNA. Using TRIzol LS Reagent allows you to increase the output of miRNA by 2,7 times compared with the use of TRIzol Reagent.