ScholarLens Back to search
2011•Journal of the South China Agricultural UniversityRequires access

Analysis of Expression Profile of OsUgp1 Gene by Promoter-GUS Chimeric Expression

Zhenxia Su, Ou GuangLiang, Yiheng Li, Mu Hong

Open publisher page 0 citations

Abstract

Promoter of one UDP-glucose pyrophosphorylase gene(OsUgp1) was cloned,fused with β-glucuronidase(GUS) reporter gene and introduced intro rice.Histochemical analysis of GUS activity was used to detect the GUS expression in transgenic rice.The result showed that GUS expressed in root,stem,leave,palea,lemma,ovary,stigma,anther at meiosis stage,developing endosperm and embryo.The expression of OsUgp1 gene in developing seed was compared with that of OsUgp2 gene.An eminent difference between OsUgp1 gene and OsUgp2 gene was found.OsUgp1 gene expressed in endosperm and embryo in the seed 5-20 d after flowering,but OsUgp2 gene almost did not express.The number and location of the cis-element related to endosperm-specific expression were analyzed in the two promoter regions,and a possible molecular mechanism of the divergent expression of two homologous genes was discussed.

About this research paper

What this paper is about

Promoter of one UDP-glucose pyrophosphorylase gene(OsUgp1) was cloned,fused with β-glucuronidase(GUS) reporter gene and introduced intro rice.Histochemical analysis of GUS activity was used to detect the GUS expression in transgenic rice.The result showed that GUS expressed in root,stem,leave,palea,lemma,ovary,stigma,anther at meiosis stage,developing endosperm and embryo.The expression of OsUgp1 gene in developing seed was compared with that of OsUgp2 gene.An eminent difference between OsUgp1 gene and OsUgp2 gene was found.OsUgp1 gene expressed in endosperm and embryo in the seed 5-20 d after flowering,but OsUgp2 gene almost did not express.The number and location of the cis-element related to endosperm-specific expression were analyzed in the two promoter regions,and a possible molecular mechanism of the divergent expression of two homologous genes was discussed.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Promoter of one UDP-glucose pyrophosphorylase gene(OsUgp1) was cloned,fused with β-glucuronidase(GUS) reporter gene and introduced intro rice.Histochemical analysis of GUS activity was used to detect the GUS expression in transgenic rice.The result showed that GUS expressed in root,stem,leave,palea,lemma,ovary,stigma,anther at meiosis stage,developing endosperm and embryo.The expression of OsUgp1 gene in developing seed was compared with that of OsUgp2 gene.An eminent difference between OsUgp1 gene and OsUgp2 gene was found.OsUgp1 gene expressed in endosperm and embryo in the seed 5-20 d after flowering,but OsUgp2 gene almost did not express.The number and location of the cis-element related to endosperm-specific expression were analyzed in the two promoter regions,and a possible molecular mechanism of the divergent expression of two homologous genes was discussed.

Key concepts: Endosperm, Biology, Gene, GUS reporter system, Chimeric gene, Gene expression, Reporter gene, Embryo

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Analysis of Expression Profile of OsUgp1 Gene by Promoter-GUS Chimeric Expression — Research Paper | ScholarLens