Brachycephalic airway obstruction syndrome in dogs. A review of 28 cases (2008–2014)

Chronically healing wounds affect many animals seen in general practice.Routinely, microbiological culturing is used to profile the bacterial species present in these wounds.Studies into the microbiota of these types of wounds in human medicine have shown that culture may be insufficient for detecting the extent of the bacterial species present.Culturefree 16S ribosomal RNA (rRNA) sequencing, being more sensitive than culture, may give a more accurate picture of the bacteria present.This study compares the results of culture and molecular testing in chronic wounds in animals. METHODSamples from 10 chronic wounds (6 dogs and 4 cats) underwent culture and 16S rRNA sequencing.Genomic DNA was isolated and the 16S rRNA V3-V4 region was amplified by PCR.Subsequently, libraries of the genomic DNA were constructed for sequencing on the Illumina MiSeq platform.The resulting sequence reads were Canine raw meat diets and antimicrobial resistant E. coli: is there a link?