2013•Science and Technology Development JournalOpen access

Study on synthesis of colloidal gold nanoparticles and preparation of antiE. coli O157 monoclonal antibody conjugated nanoparticles

Thanh Vo Ke Ngo, Giang Dang Nguyen, Quang Vinh Lam, Đạt Thành Huỳnh

Abstract

The present paper describes the preparation of colloidal gold nanoparticles by a chemical reduction method, using some chemical compounds such as sodium citrate (Na3Ct), chloroauric acid (HAuCl4.3H2O) and anti-E. coli O157 monoclonal antibodyconjugated gold nanoparticles. The nanoparticles were characterized by several techniques including Ultraviolet and Visible Absorption Spectroscopy (UV-Vis), Transmission Electron Microscopy (TEM). The UV-Vis confirmed that the concentration of Na3Ct has a major effect on the size of the synthesized gold nanoparticles, and TEM images showed that the size of gold particles is in the range of 10-20 nm. In addition, the binding of the antibody-gold conjugates to E. coli O157 was also investigated using the same characterization means

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Key concepts: Chloroauric acid, Colloidal gold, Nanoparticle, Conjugated system, Ultraviolet visible spectroscopy, Transmission electron microscopy, Sodium citrate, Nuclear chemistry