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Construction and expression of human anti-HAV Fab eukaryotic expression vector

Changming Yu, Jing Xu, Guoqi Liu, Yigang Tong, Hongbin Song, Zhikai Xu, Haitao Wang

Abstract

Fd and light chain genes were ligated to their signal peptide sequences and inserted into pCdhfr1 or pCDNA3.1, respectively. The recombinant plasmids were co-transfected into CHO cells, and resistant clones were obtained by G418 selection. The expression of Fab gene was detected by ELISA. Recombinant plasmids were successfully constructed. The plasmid-transfected CHO cells were obtained by co-transfection and G418 selection. ELISA confirmed the expression of anti-HAV Fab in culture medium.

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Available abstract

Key concepts: Plasmid, Recombinant DNA, Transfection, Molecular biology, Gene, Biology, Expression vector, Vector (molecular biology)