DETEKSI VARIASI TIGA JENIS KODOK (BUFONIDAE)DENGAN METODE PCR-RFLP

Research about PCR-RFLP gene 16S rRNA in toad (Bufonidae) using restriction ezymes has conducted from October 2016 to January 2017. The aim of this research is to detect variation from three species of Bufonidae using PCR-RFLP method gene 16S rRNA. DNA sampel was isolated dan amplified by PCR process. RFLP was done using two restriction enzymes, MseI (Tru1I) and HphI. For restriction enzyme MseI produce different restriction sites and fragment that are vary in length for three species of toad that makes us able to detect variation. RFLP with restriction enzymes HphI produce restriction sites and fragments that are equal in length so it can’t be use to detect variation between three species of toad from genus Duttaphrynus and Phrynoidis \n \nKeywords: Bufonidae, PCR-RFLP, 16S rRNA, primer, restriction enzyme