Man Li, Qinye Song, Yanqin Li, Zuo Yuzhu, Tanqing Li
Abstract
猪圆环病毒2型(PCV2)是猪圆环病毒相关疾病(PCVAD)的必要病原,对养猪生产构成了严重威胁,但当前缺乏有效的防治手段.为了探索治疗PCVAD的新途径,分别针对PCV2的病毒复制相关蛋白基因(rep)与衣壳蛋白基因(cap),构建了PCV2特异的siRNA表达质粒(pGensil-R259、pGensil-C297和pGensilC490)和PCV2非特异的阴性对照质粒(pGensiI-SCR...
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猪圆环病毒2型(PCV2)是猪圆环病毒相关疾病(PCVAD)的必要病原,对养猪生产构成了严重威胁,但当前缺乏有效的防治手段.为了探索治疗PCVAD的新途径,分别针对PCV2的病毒复制相关蛋白基因(rep)与衣壳蛋白基因(cap),构建了PCV2特异的siRNA表达质粒(pGensil-R259、pGensil-C297和pGensilC490)和PCV2非特异的阴性对照质粒(pGensiI-SCR...
Key concepts: Porcine circovirus, Plasmid, Biology, Virology, Circovirus, Replication (statistics), Small interfering RNA, Molecular biology