2019•Proceedings of the National Academy of SciencesOpen access
Microbe-focused glycan array screening platform
Andreas Geissner, Anika Reinhardt, Christoph Rademacher, Timo Johannssen, João T. Monteiro, Bernd Lepenies, Michel Thépaut, Franck Fieschi, Jana Mrázková, Michaela Wimmerová, Frank Schuhmacher, Sebastian Götze, Dan Grünstein, Xiaoqiang Guo, Heung Sik Hahm, Jeyakumar Kandasamy, Daniele Leonori, Christopher E. Martin, Sharavathi G. Parameswarappa, Sandip Pasari, Mark K. Schlegel, Hidenori Tanaka, Guozhi Xiao, You Yang, Claney L. Pereira, Chakkumkal Anish, Peter H. Seeberger
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Abstract
Interactions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein-glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.