2013•Al-Qadisiyah Journal of Veterinary Medicine SciencesOpen access

Streptococcus agalactiae mastitis of bovine detection by Polymerase Chain Reaction (PCR) test in AL-Diwanyia province

Gh. K. A. Al-kuzaay, Qassim Haleem Kshash

Abstract

This study was conducted for exam 348 milk samples from (clinically mastitic and other healthy cows) in many areas in AL-Diwanyia province by using CMT and bacteriological testing , which appeared that (64.9%) as percentage of mastitis ( clinically 15.9% , subclinically 84.0% ) Streptococcus agalactiae mastitis 13.2% ( 26.6% clinically , 73.3 % subclinicaly) diagnose by PCR assay by using specific primer (16SrRNA). Streptococcus agalactiae (30 isolates) after classical methods applied for streptococcus agalactiae identification (86 isolates).

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Key concepts: Streptococcus agalactiae, Mastitis, Microbiology, Polymerase chain reaction, Primer (cosmetics), Streptococcus, Biology, Medicine