Synthesis of Silver Nanoparticles and the Development in Analysis Method

Synthesis of silver nanoparticles and the development of silver nanoparticles in analytical method has been done. Silver nanoparticles was prepared by chemical reduction method, with variant of silver nitrate concentration and stirring time to result te best silver nanoparticles. The objective of this research is to determain the optimum condition for synthesis of silver nanoparticles and the ability of silver nanoparticles for a colorimetric sensor. The silver nanoparticles were characterized by UV-Visible and Paricle Size Analyzer (PSA). The result of characterized by UV-visible specthrophotometer showed that the UV-vis absorption spectra of silver nanoparticles with difference silver nitrate concentration gave a difference size of silver nanoparticles and the stirring time controlled the stable colloidal of silver nanoparticles. The size distribution of silver nanoparticles were confirmed by using the Paricle size analyzer (PSA). We have applied colloid silver nanoparticles as colorimetric sensor of Sialic acid and Melamine, the presence of Sialic acid and Melamine induces the aggregation of silver nanoparticles, accompanied by a color change from yellow to red-purplish (Sialic acid) and yellow to brown (melamine)