2018•DOAJ (DOAJ: Directory of Open Access Journals)Open access
Protective effect of silver nano particles against ovarian polycystic induced by morphine in rat
Elham Salami, Manizheh Karami, Ameneh Jafaryan Dehkordi, Mohammadreza Jalali Nadoushan, Abazar Hajnorouzi
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Abstract
Background and Objective: Morphine can cause harmful effects in the ovaries. The silver nanoparticles (Ag-NPs) used in health care because of antimicrobial properties, can diffuse into the brain blood barrier. This study investigated the protective effect of Ag-NPs on the induction of polycystic ovary (PCO) due to injection of morphine intra-ventromedial hypothalamus (intra-VMH) of rat compared with the animal receiving the drug (10-100 mg/kg) intraperitoneally (i.p.) twice daily for 7 days to lead to addiction. Materials and Methods: The rats (bought of Pasture Institute of Iran weighing 200-250 g) were housed under standard conditions and fed ad libitum. They were randomly divided to addict to drug or morphine (0.001 to 0.4 μg/rat) receiving into the VMH (AP: -1.92) a week after stereotaxic surgery. Ag-Nps (0.01, 0.001 and 0.0001 μg/rat) were administered intra-VMH alone or prior to morphine effective dose (0.4 μg/rat). Control group was given only saline. By the end of the treatment, the animals’ ovaries and/or brain were dissected and studied histopathologically. The ovaries were also checked by the marker of the NO, NADPH-diaphorase. Results: All experimental rats’ ovaries treated morphine showed polycystic characteristics and the NO activation was evidenced in the ovaries in the comparison with the saline group (p