Comparative evaluation of mesenchymal stem cells cultivation methods on biodegradable L-polylactide scaffold for the creation of tissue-engineered vascular implant

Cell integration and cultivation on the matrix are the key processes in the development of a tissue-engineered vascular implant (TEVI) based on a biodegradable polymer scaffold. The aim of this researh is to identify the optimal method of mesenchymal stem cells cultivation on the tubular biodegradable scaffold from L-polylactide. The comparative evaluation of the static and dynamic cultivation methods was performed. Proposed method of combining filtration method of cell integration and dynamic cultivation has proved its higher efficiency and is suitable for further development of TEVI.