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2018•Al-Qadisiyah Journal of Veterinary Medicine SciencesOpen access

Isolation and identification of Salmonella serotypes in poultry

Batool Kadhim Meteab, Alaa Abed

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Abstract

Our study was designed to investigate the prevailing serotypes of Salmonella in chickens and the rate of isolation of each serotype. Between 27 September 2014 and 15 February 2015, a total of 200 samples were collected from cloacal swabs and cecal contents from different sources and ages at Al-Diwaniyah Province. The bacterial culture was carried out by using different culture media such as Salmonella-Shigella agar (SS), Xylose Lysine Deoxycholate Agar (XLD) and Chrome agar Salmonella (CAS), the serotype was determined by confirmative bio-chemical essays using the Vitek-2 system. The current study was able to identify three serotypes as following: S. enteritidis, S. Paratyphi B and S. typhimurium with isolation rate of 10%, 6.5%, and 4.5% respectively.

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What this paper is about

Our study was designed to investigate the prevailing serotypes of Salmonella in chickens and the rate of isolation of each serotype. Between 27 September 2014 and 15 February 2015, a total of 200 samples were collected from cloacal swabs and cecal contents from different sources and ages at Al-Diwaniyah Province. The bacterial culture was carried out by using different culture media such as Salmonella-Shigella agar (SS), Xylose Lysine Deoxycholate Agar (XLD) and Chrome agar Salmonella (CAS), the serotype was determined by confirmative bio-chemical essays using the Vitek-2 system. The current study was able to identify three serotypes as following: S. enteritidis, S. Paratyphi B and S. typhimurium with isolation rate of 10%, 6.5%, and 4.5% respectively.

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Available abstract

Our study was designed to investigate the prevailing serotypes of Salmonella in chickens and the rate of isolation of each serotype. Between 27 September 2014 and 15 February 2015, a total of 200 samples were collected from cloacal swabs and cecal contents from different sources and ages at Al-Diwaniyah Province. The bacterial culture was carried out by using different culture media such as Salmonella-Shigella agar (SS), Xylose Lysine Deoxycholate Agar (XLD) and Chrome agar Salmonella (CAS), the serotype was determined by confirmative bio-chemical essays using the Vitek-2 system. The current study was able to identify three serotypes as following: S. enteritidis, S. Paratyphi B and S. typhimurium with isolation rate of 10%, 6.5%, and 4.5% respectively.

Key concepts: Isolation (microbiology), Serotype, Salmonella, Identification (biology), Biology, Microbiology, Virology, Bacteria

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