Patterns of Phenol in a Continuous Toxicity Monitoring System Using Bioluminescent Bacteria
Kil-Soo Lee, Chang-Keun Wang, Keum-Yong Hong, Jungwon Lee
Abstract
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Kil-Soo Lee, Chang-Keun Wang, Keum-Yong Hong, Jungwon Lee
Abstract
Open-access reader
The purpose of this study is to evaluate effect of phenol toxicity of Vibrio fischeri using MicroTox Ⓡ CTM.A continuous flow system for biomonitoring tool has been used to assess the toxicity of phenol.The bioluminescence inhibition of different dillutions of phenol (1.5, 2.0, 2.5, 3.0 mg/L) with deionized water for reference was evaluated from 3.5 to 97.4% and the EC20 and EC50 from inhibition curve were estimated in 1.7 and 2.2 mg/L, respectively.Meanwhile, the bioluminescence inhibition of mixtures of phenol (2.0, 3.0, 5.0, 7.0 mg/L) with effluent wastewater for reference was evaluated from 5.4 to 99.2% and the EC20 and EC50 from inhibition curve were estimated in 2.9 and 4.4 mg/L, respectively.Using MicroTox Ⓡ CTM provides a simple, rapid and sensitive method for accessing the toxic risks posed by phenol to aquatic ecosystems and has practical applications for municipal and industrial wastewater management and screening of contaminants in fresh water streams and lakes.
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The purpose of this study is to evaluate effect of phenol toxicity of Vibrio fischeri using MicroTox Ⓡ CTM.A continuous flow system for biomonitoring tool has been used to assess the toxicity of phenol.The bioluminescence inhibition of different dillutions of phenol (1.5, 2.0, 2.5, 3.0 mg/L) with deionized water for reference was evaluated from 3.5 to 97.4% and the EC20 and EC50 from inhibition curve were estimated in 1.7 and 2.2 mg/L, respectively.Meanwhile, the bioluminescence inhibition of mixtures of phenol (2.0, 3.0, 5.0, 7.0 mg/L) with effluent wastewater for reference was evaluated from 5.4 to 99.2% and the EC20 and EC50 from inhibition curve were estimated in 2.9 and 4.4 mg/L, respectively.Using MicroTox Ⓡ CTM provides a simple, rapid and sensitive method for accessing the toxic risks posed by phenol to aquatic ecosystems and has practical applications for municipal and industrial wastewater management and screening of contaminants in fresh water streams and lakes.
Key concepts: Effluent, Vibrio, Bioassay, Wastewater, Phenol, Environmental chemistry, EC50, Bioluminescence