The use of the three-dimensional reconstruction method in the morphological investigation of the left ventricle chordae tendineae of the human's heart

Objective — to determine the properties of chordae tendineae of the human’s heart left ventricle by a comlex of morphological methods of investigation using the method of three-dimensional reconstruction.Material and methods. The macroscopic method, methods of light microscopy and three-dimensional reconstruction were used for the study. The material for the study was the chordae tendineae of the left ventricles of 45 hearts of people of mature age.Results. By using the macroscopic method and method of light microscopy, a typical mitral valve chordae tendineae, that in the form of strands extend from the apex of papillary muscles and combined with valve’s cusps, and false chordae tendineae, that unlike typical ones, do not attach to the mitral valve cusps, but are ectopically fixed to the free walls of the left ventricle, interventricular septum or papillary muscles were studied. The light microscopy method showed that the typical chordae tendineae formed by one centrally located collagen core, surrounded by the peripherally located collagen-elastic layer and lined by the endothelium outside. Based on the studies, the false chordae tendineae by histological structure of their core have been divided into the fibrous, fibro-muscular and muscular types. The reproduced models of the spatial organization of the typical and false chordae tendineae of the left ventricle of the human heart reproduced the outlines and character of the surface of the chords, and showed the spatial interaction of the structural components of their inner contents with the calculation of the parameters of their relative area.