2018Unpublished venueRequires access

Multi-dimensional digital holographic microscopy

Osamu Matoba, Xiangyu Quan, Yasuhiro Awatsuji, Manoj Kumar

Open publisher page 1 citations

Abstract

In this paper, we present a multi-dimensional digital holographic microscopy for biological applications. First, we present our concept of an optical microscopy that can produce both phase and fluorescence images. Then, a technique is demonstrated to obtain the three-dimensional distribution of fluorescence beads by a common-path off-axis incoherent digital holographic microscopy. Experimental verification of the proposed system on microscopic samples and the reconstructed image quality is discussed.

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What this paper is about

In this paper, we present a multi-dimensional digital holographic microscopy for biological applications. First, we present our concept of an optical microscopy that can produce both phase and fluorescence images. Then, a technique is demonstrated to obtain the three-dimensional distribution of fluorescence beads by a common-path off-axis incoherent digital holographic microscopy. Experimental verification of the proposed system on microscopic samples and the reconstructed image quality is discussed.

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OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

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Available abstract

In this paper, we present a multi-dimensional digital holographic microscopy for biological applications. First, we present our concept of an optical microscopy that can produce both phase and fluorescence images. Then, a technique is demonstrated to obtain the three-dimensional distribution of fluorescence beads by a common-path off-axis incoherent digital holographic microscopy. Experimental verification of the proposed system on microscopic samples and the reconstructed image quality is discussed.

Key concepts: Digital holographic microscopy, Microscopy, Holography, Digital holography, Fluorescence microscope, Optical microscope, Optics, Materials science

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