Multi-dimensional digital holographic microscopy
Osamu Matoba, Xiangyu Quan, Yasuhiro Awatsuji, Manoj Kumar
Abstract
Osamu Matoba, Xiangyu Quan, Yasuhiro Awatsuji, Manoj Kumar
Abstract
In this paper, we present a multi-dimensional digital holographic microscopy for biological applications. First, we present our concept of an optical microscopy that can produce both phase and fluorescence images. Then, a technique is demonstrated to obtain the three-dimensional distribution of fluorescence beads by a common-path off-axis incoherent digital holographic microscopy. Experimental verification of the proposed system on microscopic samples and the reconstructed image quality is discussed.
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In this paper, we present a multi-dimensional digital holographic microscopy for biological applications. First, we present our concept of an optical microscopy that can produce both phase and fluorescence images. Then, a technique is demonstrated to obtain the three-dimensional distribution of fluorescence beads by a common-path off-axis incoherent digital holographic microscopy. Experimental verification of the proposed system on microscopic samples and the reconstructed image quality is discussed.
Key concepts: Digital holographic microscopy, Microscopy, Holography, Digital holography, Fluorescence microscope, Optical microscope, Optics, Materials science