Comparison of Anti-Bacterial Activity Using Methanolic and Ethanolic Extract of Houttuynia Cordata Thunb. (Yu Xing Cao)

This study was conducted to know and compare the anti-bacterial activity of methanolic and ethanolic extracts of Houttuynia cordata Thunb (HCT) against Staphylococcus aureus, Bacillus subbtilis, Escherichia coli and Pseudomonas aeruginosa. Microorganisms were grown in culture medium (Nutrient Agar). Disc diffusion method for anti-microbial susceptibility testing was performed according to the standard Kirby Bauer method. The Smith filter paper 102 discs of 6mm sizes was impregnated with the 100mg/ml of plant extract which were placed on Nutrient Agar plates seeded with bacterial cultures. Gentamicin (10ul) was used as positive control while methanol and ethanol were used as negative control. The anti-bacterial activities were assessed by the absence or presence of zone of inhibition after incubating at 37°C for 24 hours in inverted position. In this study, the methanolic and ethanolic extract did not showed any zone of inhibition.