EFFECTS OF CULTIVATION CONDITIONS ON THE BIOSYNTHESIS OF BACTERIAL NANOCELLULOSE

This paper describes conditions for the cultivation of bacterial nanocellulose using various primary producers. Research into parameters determining a high yield in the production of bacterial nanocellulose is highly relevant due to an increased demand in this product in various industries. Key parameters that affect the growth of cellulose-synthesizing bacteria and the biosynthesis of bacterial nanocellulose include the following: the concentration of the carbon source in the nutrient solution; aeration; cultivation temperature; active acidity level. The concentration of reducing substances in the nutrient solution can range from 6 to 100 g/l. The concentration of dissolved oxygen in the nutrient solution can be considered as a limiting factor for all cellulose-synthesizing microorganisms. It is shown that the temperature range for the bacterial nanocellulose biosynthesis can vary from 25 to 33°C for various primary producers. PH values that provide a maximal yield of bacterial nanocellulose are determined to range from 4 to 6.5 for various primary producers. The literature review has proven the importance of a case-by-case approach when selecting cultivation conditions for every primary producer so as to maximize the bacterial nanocellulose yield.