Physical parameter and microbial screening in Lata Janggut, Jeli, Kelantan

Water microbe can be found widely in freshwater, ocean, lake or reservoir. Upstream \nLata Janggut, Jeli, Kelantan is chosen as the study area to culture and isolate the \nmicroorganism from freshwater. Seven isolates microorganism were obtained. Based on \nmorphology characteristics which are shape margin and elevation, bacteria were \ncharacterized. Result from Gram staining for the sample showed all the bacteria were \nGram positive bacteria. The shapes of bacteria obtained are coccus, dipplobacilli, \ncocobacillus and bacillus. Seven isolates microorganism obtains undergo DNA \nextraction to obtain DNA.16s rRNA universal primer was used to amplify the copy of \nDNA by performing Polymerase Chain Reaction (PCR). The result from amplifying the \ncopy of DNA showed multiple bands. Optimizations of primer design can reduce \nmultiple bands. Number of control also should be considered when performing PCR.