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2018•Journal of ApicultureRequires access

Detection of Sugar Cane (Saccharum officinarum)-specific Gene from Sugar and Sugar-honey

Byounghee Kim, So-Min Kim, Moon Jung Kim, Jung Min Kim, Truong A Tai, Byoung-Su Yoon

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Abstract

Sugar cane-specific gene could be successfully amplified with DNAs isolated from sugar or sugarhoney using Saccharum officinarum-specific Ultra-Rapid or conventional PCR. Specificity of PCR products from sugar or sugar-honey was verified by nested PCR and DNA sequencing. This PCR could be applied to a quantitative analysis for honey-evaluation. In our knowledge, it is first report that sugar cane-specific sequence could be detected from sugar-honey or sugar itself, and that sugar-honey could be evaluated by genetic examination.

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What this paper is about

Sugar cane-specific gene could be successfully amplified with DNAs isolated from sugar or sugarhoney using Saccharum officinarum-specific Ultra-Rapid or conventional PCR. Specificity of PCR products from sugar or sugar-honey was verified by nested PCR and DNA sequencing. This PCR could be applied to a quantitative analysis for honey-evaluation. In our knowledge, it is first report that sugar cane-specific sequence could be detected from sugar-honey or sugar itself, and that sugar-honey could be evaluated by genetic examination.

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Available abstract

Sugar cane-specific gene could be successfully amplified with DNAs isolated from sugar or sugarhoney using Saccharum officinarum-specific Ultra-Rapid or conventional PCR. Specificity of PCR products from sugar or sugar-honey was verified by nested PCR and DNA sequencing. This PCR could be applied to a quantitative analysis for honey-evaluation. In our knowledge, it is first report that sugar cane-specific sequence could be detected from sugar-honey or sugar itself, and that sugar-honey could be evaluated by genetic examination.

Key concepts: Saccharum officinarum, Sugar, Sugar cane, Saccharum, Biology, Gene, Polymerase chain reaction, Cane

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