OPTIMIZATION OF ENVIRONMENTAL PARAMETERS FOR MAXIMUM TANNASE PRODUCTION FROM CASHEW HUSK

Tannase production under solid-state fermentation was investigated using isolated Aspergillus oryzae. Among all agro-industrial waste material evaluated, cashew husk supported maximum tannase production. The metabolic processes of microorganisms are influenced by changes in parameters like Temperature, pH, incubation time, humidity etc., which are very specific for a particular organism. Microbial synthesis of enzymes in a SSF process are also affected by factors like particle size of substrate, water content, relative humidity, type and size of inoculum, control of temperature, period of cultivation, etc. Biotransformation of cashew husk tannin to gallic acid by SSF is also influenced by all the factors affecting tannase production, since the synthesized enzyme causes the breakdown of tannin to gallic acid and glucose.