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2018•Blucher Chemical Engineering ProceedingsOpen access

CULTIVATION OF Bacillus thuringiensis var. israelensis H14 IN BIOREACTOR FOR BIOLOGICAL CONTROL OF Aedes aegypti LARVAE

L. F. A. MELO, Amanda Cabral, Ana Claudia A. Melo, Maria Alice Varjal de Melo-Santos, L. FINKLER, Christine Lamenha Luna-Finkler

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Abstract

The aim of this study was the cultivation of Bacillus thuringiensisvar. israelensis (Bti) H14 for the biological control of Aedes aegypti. Bti cultureswere cultivated in a bioreactor, and the growth kinetics, toxin concentration andlarvicidal activity were evaluated. The results showed that the bacterialfermentates produced on average 0.62 g/L of toxin (LC50 2.25 mg/L and LC903.82 mg/L). Upon concentration of the biomass, the toxin yield was almostthree-fold higher (1.64 g/L), also resulting in an increase in the bioactivity(LC50 0.46 mg/L and LC90 0.84 mg/L). The fermentation conditions employedwere suitable for the cultivation of Bti, generating batches with highconcentration of cells, short time required for sporulation and adequateproduction of toxins with confirmed biolarvicidal activity.

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What this paper is about

The aim of this study was the cultivation of Bacillus thuringiensisvar. israelensis (Bti) H14 for the biological control of Aedes aegypti. Bti cultureswere cultivated in a bioreactor, and the growth kinetics, toxin concentration andlarvicidal activity were evaluated. The results showed that the bacterialfermentates produced on average 0.62 g/L of toxin (LC50 2.25 mg/L and LC903.82 mg/L). Upon concentration of the biomass, the toxin yield was almostthree-fold higher (1.64 g/L), also resulting in an increase in the bioactivity(LC50 0.46 mg/L and LC90 0.84 mg/L). The fermentation conditions employedwere suitable for the cultivation of Bti, generating batches with highconcentration of cells, short time required for sporulation and adequateproduction of toxins with confirmed biolarvicidal activity.

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Available abstract

The aim of this study was the cultivation of Bacillus thuringiensisvar. israelensis (Bti) H14 for the biological control of Aedes aegypti. Bti cultureswere cultivated in a bioreactor, and the growth kinetics, toxin concentration andlarvicidal activity were evaluated. The results showed that the bacterialfermentates produced on average 0.62 g/L of toxin (LC50 2.25 mg/L and LC903.82 mg/L). Upon concentration of the biomass, the toxin yield was almostthree-fold higher (1.64 g/L), also resulting in an increase in the bioactivity(LC50 0.46 mg/L and LC90 0.84 mg/L). The fermentation conditions employedwere suitable for the cultivation of Bti, generating batches with highconcentration of cells, short time required for sporulation and adequateproduction of toxins with confirmed biolarvicidal activity.

Key concepts: Aedes aegypti, Bacillus thuringiensis, Bioreactor, Toxin, Biomass (ecology), Biological pest control, Biology, Fermentation

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