Influence of Gamma Irradiation on Localization of Enzymatic Activity During Spermatogenesis of Green Vegetable Stink Bug Nezara viridula (Hemiptera: Pentatomidae)

Nezara viridula which is serious pentatomid pest for many agricultural crops had irradiated with 40 Gray (Gy) dose of gamma radiation. The ultrastructure and cytochemical studies were carried out to evaluate the activities of acid phosphatase (ACP) and gloucose-6-phosphatase (G-6-PDH) enzymes, during the spermatogenesis of non-irradiated and irradiated adult males. These studies were emphasis on the early and late spermatid stage and sperm flagellum, which involve the axoneme and mitochondria derivatives. The reaction products of (ACP) activity was associated with Golgi complex and nuclear membrane, which are relatively at the same level for both control and irradiated individual at early spermatid stage. The localization of (ACP) activity on late spermatid and spermatozoan flagellum showing strong reaction in the endoplasmic reticulum cisternae surrounding the axoneme and tail elements and was also observed on the mitochondria derivatives. The reaction products were approximately weak on irradiated male. Glucose-6-Phosphatase (G-6-PDH) was found labelling the nuclear envelope in irradiated early spermatid stage, however it was shown negative reaction in control individuals. The (G-6-PDH) was found mainly in the plasma membrane of sperm flagellum, endoplasmic cisternae surrounding axoneme and outer boarder of mitochondria derivatives. The activity of this enzyme was similar in both control and irradiated N. viridula.