2018Diyala Journal of MedicineOpen access

Isolation and Purification of metalloprotease produced by Aeromonas hydrophila

Muhannad Moeen Abdallah, Tahreer Hadi Saleh

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Abstract

Background: The bacteria Aeromonas hydrophila was isolated from clinical specimens responsible of causing diarrhea in children and adults. Objective:To purify metalloprotease enzyme produced by Aeromonas hydrophila. Patients and Methods: This study involved 150 stool samples collected from patients suffering from diarrhea. Results: Eight isolate of Aeromonas hydrophila was detected and metalloprotease was purified with 11.06 U/ml of enzyme activity. Conclusion: The bacteria have ability to produce metalloprotease and enzyme was fully purified in three steps of purification.

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Background: The bacteria Aeromonas hydrophila was isolated from clinical specimens responsible of causing diarrhea in children and adults. Objective:To purify metalloprotease enzyme produced by Aeromonas hydrophila. Patients and Methods: This study involved 150 stool samples collected from patients suffering from diarrhea. Results: Eight isolate of Aeromonas hydrophila was detected and metalloprotease was purified with 11.06 U/ml of enzyme activity. Conclusion: The bacteria have ability to produce metalloprotease and enzyme was fully purified in three steps of purification.

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Available abstract

Background: The bacteria Aeromonas hydrophila was isolated from clinical specimens responsible of causing diarrhea in children and adults. Objective:To purify metalloprotease enzyme produced by Aeromonas hydrophila. Patients and Methods: This study involved 150 stool samples collected from patients suffering from diarrhea. Results: Eight isolate of Aeromonas hydrophila was detected and metalloprotease was purified with 11.06 U/ml of enzyme activity. Conclusion: The bacteria have ability to produce metalloprotease and enzyme was fully purified in three steps of purification.

Key concepts: Aeromonas hydrophila, Metalloproteinase, Aeromonas, Microbiology, Bacteria, Isolation (microbiology), Enzyme, Diarrhea

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