Phytochemical Analysis, Determine the Content of Total Polyphenols, Total Flavonoids and Antioxidant Activity of Leaves from Syrian Pinus Brutia

The aim of this study was to phytochemical screening and determine the content of total polyphenols,total Flavonoids and antioxidant activity of methanol(80%) extract, dichloromethane, ethyl acetate and butanol extracts of leaves from pinus brutia ,Quantitative estimation of phenols and flavonoids have been studied using Folin-Ciocalteu method to estimate phenolic conten , method of Aluminum chloride chemical detector for determination Flavonoid content for both methanol(80%) extract, dichloromethane extract, ethyl acetate extract and systemic butanol extract.The antioxidant activity of the previous extracts was studied using the phosphate molybdate method and the results showed that the highest efficacy of the methanol 80% extract .The results showed that the phenolic content of the methanol extract was the largest (429.44 mg gallic acid per gram of dry extract), The flavonoid content of the methanol extract showed the highest value compared with the previous extracts (98.27 mg equivalent for quercetin for each gram of dry extract).The antioxidant activity of the methanol extract was the largest(113.32mg equivalent for ascorbic acid for each gram of dry extract).