2016•Molecular Genetics Microbiology and Virology (Russian)Requires access
Development of the method for identification and genotyping of the bacteria Pasteurella multocida and Mannheimia haemolytica on the basis of the pcr and phylogenetic analysis of bacterial cultures isolated from cattle
A.V. Nefedchenko, A. N. Shikov, A.G. Glotov, Т.I. Glotova, В. А. Терновой, А. П. Агафонов, Sergeev An, Н. А. Донченко
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Abstract
The results of development of a method for detection and genotyping of the bacteria Pasteurella multocida capsular five groups and Mannheimia haemolytica Al based on the multiplex polymerase chain reaction (PCR) with electrophoretic detection are submitted. Diagnostic sensitivity of the developed method was 103 CFU/ml in the study of the pure cultures and 105 CFU/g in the study of biological material. A study of 260 samples of biological material from infected animals revealed Pasteurella multocida in 50.0%, and Mannheimia haemolytica in 11.2% of the investigated samples. Circulation among the tested livestock of capsular groups B and E of Pasteurella multocida was not revealed. The majority of the tested samples contained group A, in some cases, group D, and, in one case, group F. On the basis of the phylogenetic analysis circulation of two different genetic types of Pasteurella multocida of the capsular group A was revealed.